DENDRITICS
 
Research topic > Flow cytometry
 
Flow cytometry  print this page
 

1.DENDRITICS monoclonal antibodies are used for Flow cytometry :

  • surface                                                                             
  • intracellular
  • fluorophores: Alexa-fluor™(488, 546, 647)
  • conjugate-Biotin

Alexa-fluor™ dyes are a family of fluorescent dyes with exceptional fluorescence and photostability. Alexa-fluor™ 488 conjugated antibodies are detected in FL1 with any flow cytometer equipped with an argon laser that emits at 488 nm.

Alexa-fluor546 is for applications that requires fluorescent probes that emit in orange spectrum.

Alexa-fluor647 has spectral characteristics that can be an alternative Cy5 dyes.The higher fluorescence intensity of Alexa-fluor™647 conjugates has advantages in multi-color flow cytometry studies. and are studied in FL4. They can  be excitted by excitation sources such as HeNe laser line or red diode laser emitting at 633 and 635 respectively.


 

Alexa-fluor™ is a registered trade mark of  Molecular Probes Inc. (a wholy owned subsidiary of Invitrogen Corp).
 

 

 

          Alexafluor™             research only             

 Absorption (nm)

Emit (nm)

Reported applications

488

488 519

FCM (FL1), IF

546

556 573

 IF, confocal

647

633 668

FCM (FL4)

 

 

2. DENDRITICS Cluster of Differentiation list

 
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